Brief comparison of NGS platforms

  • Short reads (SBL and SBS types)
    • SBL – Sequencing by ligation type
      • Solid [50-75 bp] (80-320 Gb)
      • BGISEQ [50-100 bp] (8-200 Gb)
    • SBS – Sequencing by synthesis type (CRT Cycle Reversible Termination)
      • Illumina [25-300 bp] (540 Mb – 900 Gb)
      • Qiagen [NA] (12 Genes, 1250 mutations)
    • SBS – Sequencing by synthesis type (SNA Single Nucleotide Addition)
      • 454 discontinued [400-1000 bp] (35-700 Mb)
      • Ion Torrent [200-400 bp] (30 Mb – 15 Gb)
  • Long reads (SM and SA types)
    • SM – Single Molecule
      • PacBio [8-20 Kb] (500 Mb – 7 Gb)
      • ONT MinION [up to 200 Kb] (1.5 Gb)
    • SA – Synthetic Approaches
      • Illumina Synthetic Long Read Sequencing Platform [~100 Kb] (64-500 Gb)
      • 10x Genomics Emulsion-based System [up to 100 Kb] (64-500 Gb)
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